Bovine Chondrocytes

 

Cartilage Chondrocytes

 

Bovine Chondrocytes are isolated from normal articular cartilage from one month old calves. The cells are cryopreserved after isolation and can be cultured and propagated through several passages. In order for chondrocytes to maintain their phenotype, i.e produce and maintain the extracellular matrix of cartilage (type II collagen and aggrecan), they must be cultured at high density (such as 1M cells/ml) or in gels or scaffolds. Chondrocytes grown at low density will lose their phenotype, and de-differentiate into fibroblast-like cells which produce type I collagen. Chondrocyte cultures are useful in vitro models for studying:

  • Cartilage regeneration and repair
  • Cytokine and growth factor effects on cartilage
  • Arthritis when co-cultured with anti-collagen antibodies and/or cells of inflammation such as activated macrophages and lymphocytes.

Catalog Number Product Name Size Price In Stock
1020-84JN07 Bovine Chondrocytes 1 M cells/vial $275.00 2
1021-269OC09 Bovine Chondrocytes 10 million cells per vial $825.00 6
1021-270OC09 Bovine Chondrocytes 10 million cells per vial $825.00 55
1021-90OC07 Bovine chondrocytes 5 million cells per vial $600.00 10

chondrocyte proliferation

Chondrocytes were thawed and plated into a 96 well plate at 100,000 cells per well. Recombinant human FGF18 was added at 100 ul per well. Cultures were incubated for 2 days prior to measuring proliferation by reduction of XTT. See more details in the protocol Chondrocyte culture.

proteoglycan synthesis

Chondrocytes were thawed and cultured in 96 well plates for 2-4 days. Culture supernatants were removed and proteoglycan in the medium was measured using DMB. In the graph below type II collagen was measured using our Type II collagen kit. For more experimental details see Measuring cartilage synthesis

 

Type II collagen synthesis align=

 

Publications

Mintz BR and Cooper JA. 2013. Hybrid Hyaluronic Acid Hydrogel/Poly (ε‐caprolactone) Scaffold Provides Mechanically Favorable Platform for Cartilage Tissue Engineering Studies Journal of Biomedical Materials Research Part A