Autoimmune T cells are believed to be the proximate cause of multiple sclerosis (MS). T cells specific for myelin antigens cause inflammatory demyelination in animal models of MS and have been extensively studied for decades. Myelin basic protein is the myelin antigen that has been most studied and MBP specific T cells have been detected in patients with MS.
The BC3 cell line was isolated from a normal 56 year old Caucasian man. In vitro stimulation of peripheral blood mononuclear cells (PBMC) with a peptide of MBP was used to select for peptide reactive T cells. The peptide used (ENPVVHFFKNIVTPRTP) is believed to be the immunodominant epitope. The cell line is likely oligoclonal and recognizes the peptide presented by HLA-DRβ1*1501 and may also recognize peptide presented by HLA-DRβ1*14, though this has not yet been confirmed. The cells also recognize the peptide FFKNIVTPRTPPPSQGK, thus the minimal epitope for this line is FFKNIVTPRTP.
The cells secrete interferon gamma, IL-13 and TNF alpha upon stimulation. They are CD4+ and, not surprisingly, FoxP3 negative.
Instructions for maintaining the cells in culture can be found at the bottom of this page. B lymphoblastoid cells from this donor are also listed below and can be used as antigen presenting cells for maintenance and testing of the T cells.
Ji N, Somanaboeina A, Dixit A, Kawamura K, et al. Small Molecule Inhibitor of Antigen Binding and Presentation by HLA-DR2b as a Therapeutic Strategy for the Treatment of Multiple Sclerosis J Immunol 2013 191:5074
|Instructions for BC3 rev 2.pdf||190.16 KB|