Astarte Biologics generates dendritic cells by first enriching monocytes then culturing in the presence of IL-4 and GM-CSF.  Cells are collected after 5-7 days of culture and characterized by flow cytometric analysis for expresssion of CD11c, CD14, CD86, CD1a, CD3 and CD19.

Dendritic cells are also tested for function in a mixed lymphocyte reaction to demonstrate their antigen presentation potential.

Dendritic cells were cultured with PBMC from two other donors for 5 days.  DC were added to wells of a 96 well plate at 25,000/well, 5,000/well or 1,000/well with a constant 250,000 PBMC per well.  Control consisted of PBMC alone.

Dendritic cells are provided at 1-2 million cells per vial. 

The link below shows the certificate of analysis for the current lot.