Astarte Biologics offers both myeloid and plasmacytoid dendritic cells. Myeloid dendritic cells are generated by first enriching monocytes then culturing in the presence of IL-4 and GM-CSF. Cells are collected after 5-7 days of culture and characterized by flow cytometric analysis for expression of CD11c, CD14, CD86, CD3 and CD19.
Plasmacytoid dendritic cells are isolated from PBMC by negative immunomagnetic selection and purity is evaluated by 3 color immunofluorescent staining for CD4, CD123 and HLA-DR antigens. We are currently out of stock but will replenish them soon.
Myeloid dendritic cells secrete IL-12 when stimulated with poly I:C as shown in the figure below. Plasmacytoid dendritic cells are known to secrete interferon alpha when stimulated with CpG oligonucleotides.
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Monocyte derived DC were thawed and added to a 96 well plate at 50,000 cells per well. Serum free medium containing GM-CSF and IL-4 was used. The next day recombinant human interferon gamma was added to half of the wells, followed by addition of poly I:C. Culture medium was collected after 48 hours and IL-12 was measured by ELISA. |
| Catalog Number | Product Name | Size | Price | In Stock | |
|---|---|---|---|---|---|
| 1010-520SE10 | Monocyte Derived Dendritic Cells | 1-2 million cells/vial | $500.00 | 2 |
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| 1010-194JN09 | Monocyte Derived Dendritic cells | 1-2 million cells/vial | $500.00 | 2 |
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Mouse Dendritic Cells are also available! See our Mouse Cell page. Click here.