Astarte Biologics generates dendritic cells by first enriching monocytes then culturing in the presence of IL-4 and GM-CSF. Cells are collected after 5-7 days of culture and characterized by flow cytometric analysis for expression of CD11c, CD14, CD86, CD1a, CD3 and CD19.
Dendritic cells are also tested for function in a mixed lymphocyte reaction to demonstrate their antigen presentation potential.
Dendritic cells were cultured with PBMC from two other donors for 5 days. DC were added to wells of a 96 well plate at 25,000/well, 5,000/well or 1,000/well with a constant 250,000 PBMC per well. Control consisted of PBMC alone.
Dendritic cells are provided at 1-2 million cells per vial.
| Catalog Number | Product Name | Size | Price | |
|---|---|---|---|---|
| 1010-195JN09 | Monocyte Derived Dendritic cells | 1-2 million cells/vial | $500.00 | |
| 1010-194JN09 | Monocyte Derived Dendritic cells | 1-2 million cells/vial | $500.00 | |
| 1010-444MY10 | Monocyte Derived Dendritic Cells | 1-2 million cells/vial | $500.00 | |
| 1010-495AU10 | Monocyte Derived Dendritic Cells | 1-2 million cells/vial | $500.00 |
Mouse Dendritic Cells are also available! See our Mouse Cell page. Click here.