Myeloid dendritic cells are generated by first enriching monocytes then culturing in the presence of IL-4 and GM-CSF. Cells are collected after 5-7 days of culture and characterized by flow cytometric analysis for expression of CD11c, CD14, CD86, CD3 and CD19.
Myeloid dendritic cells secrete IL-12 when stimulated with poly I:C as shown in the figure below.
|Monocyte derived DC were thawed and added to a 96 well plate at 50,000 cells per well. Serum free medium containing GM-CSF and IL-4 was used. The next day recombinant human interferon gamma was added to half of the wells, followed by addition of 25 ug/mL poly I:C. Culture medium was collected after 48 hours and IL-12 was measured by ELISA.|
|Catalog Number||Product Name||Donor||Size||Price||In Stock|
|1010-2017MA13||Human Monocyte Derived DC||
|1-2 M cells per vial||$500.00||24||
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