Human Dendritic Cells

Myeloid dendritic cells are generated by first enriching monocytes then culturing in the presence of IL-4 and GM-CSF.  Cells are collected after 5-7 days of culture and characterized by flow cytometric analysis for expression of CD11c, CD14, CD86, CD3 and CD19.

Myeloid dendritic cells secrete IL-12 when stimulated with poly I:C as shown in the figure below.

IL-12 secretion by DC Monocyte derived DC were thawed and added to a 96 well plate at 50,000 cells per well. Serum free medium containing GM-CSF and IL-4 was used.  The next day recombinant human interferon gamma was added to half of the wells, followed by addition of 25 ug/mL poly I:C.  Culture medium was collected after 48 hours and IL-12 was measured by ELISA.

 

Catalog Number Product Name Donor Size Price In Stock
1010-2453OC13 Monocyte Derived Dendritic Cells

1-2 M cells per vial $550.00 13
1010-2537DE13 Monocyte Derived Dendritic Cells

 

Donor:226 M1 Peptide:N/T
Blood Type:B pos CMV Antigens:neg
Age:46 Tetanus Toxoid:pos
Gender:M LPS:neg
Ethnicity:Caucasian PHA:pos
HLA-A *02 *01
HLA-B *40 *07
HLA-C *07 *03
HLA-DRβ1 *07 *03
1-2 M cells per vial $550.00 21

Mouse Dendritic Cells are also available! See our Mouse Cell page.  Click here.