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Monocyte Derived Human Dendritic Cells

Astarte Biologics generates dendritic cells by first enriching monocytes then culturing in the presence of IL-4 and GM-CSF.  Cells are collected after 5-7 days of culture and characterized by flow cytometric analysis for expresssion of CD11c, CD14, CD86, CD1a, CD3 and CD19.

 Catalog Number  Description  Cells per vial  Price per vial
 1010  Monocyte Derived Dendritic cells  1-2 million  $500.00
 

Dendritic cells are also tested for function in a mixed lymphocyte reaction to demonstrate their antigen presentation potential.

DC stimulation of TNF

Dendritic cells were cultured with PBMC from two other donors for 5 days.  DC were added to wells of a 96 well plate at 25,000/well, 5,000/well or 1,000/well with a constant 250,000 PBMC per well.  Control consisted of PBMC alone.

Dendritic cells are provided at 1-2 million cells per vial. 

The link below shows the certificate of analysis for the current lot.

   

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lot 140OC08.pdf147.44 KB