Our Monocytes are isolated by a negative selection process that prevents the activation of cells. Monocytes from Astarte Biologics function as expected:
- phagocytize yeast
- produce cytokines
- migrate in response to chemotactic agents
Under the right culture conditions, monocytes can differentiate into dendritic cells, Langerhans cells or macrophages.
| Catalog Number | Product Name | Size | Price | In Stock | |
|---|---|---|---|---|---|
| 1008-2077AP13 | Monocytes - Negatively Selected | 5-10 M cells/vial | $300.00 | 18 |
|
| 1009-2078AP13 | Monocytes - Negatively Selected | 10-20 M cells/vial | $500.00 | 6 |
|
Publications
Publications using Monocytes from Astarte Biologics:
Kaler P, Augenlicht L, Klampfer L. Activating mutations in ß-catenin in colon cancer cells alter their interaction with macrophages; the role of snail PLOS ONE 2012. http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0045462
Cai S, Batra S, Wakamatsu N, et al. NLRC4 Inflammasome-mediated production of IL-1ß modulates mucosal immunity in the lung against gram-negative bacterial infection. J Immunol 2012. http://www.jimmunol.org/content/early/2012/04/30/jimmunol.1200195.abstract
AV Finn, M Nakano, R Polavarapu, et al. Hemoglobin Directs Macrophage Differentiation and Prevents Foam Cell Formation in Human Atherosclerotic Plaques J Am Coll Cardiol published online 7 December 2011. http://content.onlinejacc.org/cgi/content/abstract/j.jacc.2011.10.852v1
K Subbaramaiah, LR Howe, P Bhardwaj, et al. Obesity is associated with inflammation and elevated aromatase expression in the mouse mammary gland Cancer Prev Res March 2011 4; 329
Xu M, et al. Hepcidin induces HIV-1 transcription inhibited by ferroportin Retrovirology 2010,7:104 full text at Wiley
Ariza M, Glaser R, Kaumaya PTP, Jones C, Williams MV. The EBV-Encoded dUTPase Activates NF-kB through the TLR2 and MyD88-Dependent Signaling Pathway. J Immunol 2009, 182: 851-859 http://www.jimmunol.org/cgi/content/abstract/182/2/851
Kaler P, Godasi BN, Augenlicht L, Klampfer L. The NF-kB/AKT-dependent Induction of Wnt Signaling in Colon Cancer Cells by Macrophages and IL-1B Cancer Microenvironment (2009) 2:69–80 http://www.springerlink.com/content/a746754h10845125/
Kaler P, Augenlicht L, Klampfer L. Macrophage-derived IL-1beta stimulates Wnt signaling and growth of colon cancer cells: a crosstalk interrupted by vitamin D3.Oncogene 2009, 28:3892-38902 http://www.nature.com/onc/journal/v28/n44/abs/onc2009247a.html
Kaler P, Galea V, Augenlicht L, Klampfer L. Tumor Associated Macrophages Protect Colon Cancer Cells from TRAIL-Induced Apoptosis through IL-1beta-Dependent Stabilization of Snail in Tumor Cells. PLoS One. 2010 5:e11700. http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.00117...
FACS Analysis
FACS analysis of a typical sample stained with antibodies to CD11c vs CD14. Samples were gated on live cells.
| IL1beta | IL6 | IL10 | IL12 | IL13 | CCL2 | CCL3 | TNFa | |
| pg/ml | pg/ml | pg/ml | pg/ml | pg/ml | pg/ml | pg/ml | pg/ml | |
| control | 1.0 | 1.2 | ND | 1.0 | 24.1 | 380.0 | 110.5 | 32.2 |
| 1 ug/mL LPS | 6.2 | 1,900.0 | 24.0 | 11.3 | 118.3 | 4,260.0 | 72,900.0 | 5,380.0 |
| 10 ug/mL LPS | 11.1 | 6,300.0 | 72.0 | 11.2 | 112.4 | 10,365.0 | 201,200.0 | 10,685.0 |
| Monocytes were thawed and cultured in 24 well plates at 1 million cells per well Culture medium was collected after 48 hours Cytokines were quantitated by Pierce Biotechnology using their Searchlight Proteome Array™ | ||||||||
Phagocytosis
Monocytes were incubated with Alexa Fluor® 595 conjugated zymosan for one hour, fixed and counterstained with Alexa Fluor® 488 conjugated phalloidin. See the phagocytosis protocol here for details of the method.
(Alexa Fluor is a registered trademark of Molecular Probes, Inc.)
Chemotaxis
Monocytes were thawed and placed into Transwells to measure migration in response to formyl-methionine-leucine-phenylalanine. Click here to see the protocol.
