Monocytes - Negative Selection
Our Monocytes are isolated by a negative selection process that prevents the activation of cells. Monocytes from Astarte Biologics function as expected:
- phagocytize yeast
- produce cytokines
- migrate in response to chemotactic agents
Under the right culture conditions, monocytes can differentiate into dendritic cells, Langerhans cells or macrophages. See our resources page for more information.
Publications using Monocytes from Astarte Biologics:
Ariza M, Glaser R, Kaumaya PTP, Jones C, Williams MV. The EBV-Encoded dUTPase Activates NF-kB through the TLR2 and MyD88-Dependent Signaling Pathway. J Immunol 2009, 182: 851-859
http://www.jimmunol.org/cgi/content/abstract/182/2/851Specifications
| Catalog Number | Product Name | Price | Size | |
|---|---|---|---|---|
| 1008-123JN08 | Monocytes - Negative Selection 123JN08 | $300.00 | 5-10 M cells /vial | |
| 1009-124JN08 | Monocytes - Negative Selection 124JN08 | $500.00 | 10-20 M cells/vial | |
| 1008-144OC08 | Monocytes- Negative Selection 144OC08 | $300.00 | 5-10 M cells/vial |
FACS Analysis
FACS analysis of a typical sample stained with antibodies to CD11c vs CD14 on (Right panel) and CD64 vs T-cell Receptor (Left panel). Samples were gated on live cells.
| IL1beta | IL6 | IL10 | IL12 | IL13 | CCL2 | CCL3 | TNFa | |
| pg/ml | pg/ml | pg/ml | pg/ml | pg/ml | pg/ml | pg/ml | pg/ml | |
| control | 1.0 | 1.2 | ND | 1.0 | 24.1 | 380.0 | 110.5 | 32.2 |
| 1 ug/mL LPS | 6.2 | 1,900.0 | 24.0 | 11.3 | 118.3 | 4,260.0 | 72,900.0 | 5,380.0 |
| 10 ug/mL LPS | 11.1 | 6,300.0 | 72.0 | 11.2 | 112.4 | 10,365.0 | 201,200.0 | 10,685.0 |
| Monocytes were thawed and cultured in 24 well plates at 1 million cells per well Culture medium was collected after 48 hours Cytokines were quantitated by Pierce Biotechnology using their Searchlight Proteome Array™ | ||||||||
Phagocytosis
Monocytes were incubated with Alexa Fluor® 595 conjugated zymosan for one hour, fixed and counterstained with Alexa Fluor® 488 conjugated phalloidin. See the phagocytosis protocol here for details of the method.
(Alexa Fluor is a registered trademark of Molecular Probes, Inc.)
Chemotaxis
Monocytes were thawed and placed into Transwells to measure migration in response to formyl-methionine-leucine-phenylalanine. Click here to see the protocol.
