Under the right culture conditions, monocytes can differentiate into dendritic cells, Langerhans cells or macrophages. See our resources page for more information.
Specifications
| Catalog Number | Description | Price |
|---|---|---|
| 1008 | Monocytes 5-10 million | $300.00 |
| 1009 | Monocytes 10-20 million | $500.00 |
FACS Analysis
FACS analysis of a typical sample stained with antibodies to CD11c vs CD14 on (Right panel) and CD64 vs T-cell Receptor (Left panel). Samples were gated on live cells.
| IL1beta | IL6 | IL10 | IL12 | IL13 | CCL2 | CCL3 | TNFa | |
| pg/ml | pg/ml | pg/ml | pg/ml | pg/ml | pg/ml | pg/ml | pg/ml | |
| control | 1.0 | 1.2 | ND | 1.0 | 24.1 | 380.0 | 110.5 | 32.2 |
| 1 ug/mL LPS | 6.2 | 1,900.0 | 24.0 | 11.3 | 118.3 | 4,260.0 | 72,900.0 | 5,380.0 |
| 10 ug/mL LPS | 11.1 | 6,300.0 | 72.0 | 11.2 | 112.4 | 10,365.0 | 201,200.0 | 10,685.0 |
| Monocytes were thawed and cultured in 24 well plates at 1 million cells per well Culture medium was collected after 48 hours Cytokines were quantitated by Pierce Biotechnology using their Searchlight Proteome Array™ | ||||||||
Phagocytosis
Monocytes were incubated with Alexa Fluor® 595 conjugated zymosan for one hour, fixed and counterstained with Alexa Fluor® 488 conjugated phalloidin. See the phagocytosis protocol here for details of the method.
(Alexa Fluor is a registered trademark of Molecular Probes, Inc.)
Chemotaxis
Monocytes were thawed and placed into Transwells to measure migration in response to formyl-methionine-leucine-phenylalanine. Click here to see the protocol.