Monocytes - Negative Selection

Our Monocytes are isolated by a negative selection process that prevents the activation of cells. Monocytes from Astarte Biologics function as expected:

  • phagocytize yeast
  • produce cytokines
  • migrate in response to chemotactic agents

Under the right culture conditions, monocytes can differentiate into dendritic cells, Langerhans cells or macrophages.


 

We're making more! The next batch is expected to be released December 9.

This product is currently out of stock.

 

Publications

Publications using Monocytes from Astarte Biologics:

Wang R, Borazjani A, Matthews AT, et al. Identification of palmitoyl protein thioesterase 1 in human THP-1 monocytes/macrophages and characterization of unique biochemical activities for this enzyme. Biochemistry 2013 abstract

RA Barve, MD Zack, D Weiss, et al. Transcriptional profiling and pathway analysis of CSF-1 and IL-34 effects on human monocyte differentiation.  Cytokine, 2013 abstract

Kaler P, Augenlicht L, Klampfer L. Activating mutations in ß-catenin in colon cancer cells alter their interaction with macrophages; the role of snail PLOS ONE 2012. http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0045462

Cai S, Batra S, Wakamatsu N, et al. NLRC4 Inflammasome-mediated production of IL-1ß modulates mucosal immunity in the lung against gram-negative bacterial infection. J Immunol 2012. http://www.jimmunol.org/content/early/2012/04/30/jimmunol.1200195.abstract

AV Finn, M Nakano, R Polavarapu, et al. Hemoglobin Directs Macrophage Differentiation and Prevents Foam Cell Formation in Human Atherosclerotic Plaques J Am Coll Cardiol published online 7 December 2011.  http://content.onlinejacc.org/cgi/content/abstract/j.jacc.2011.10.852v1

K Subbaramaiah, LR Howe, P Bhardwaj, et al. Obesity is associated with inflammation and elevated aromatase expression in the mouse mammary gland Cancer Prev Res March 2011 4; 329

Xu M, et al. Hepcidin induces HIV-1 transcription inhibited by ferroportin Retrovirology 2010,7:104    full text at Wiley

Ariza M, Glaser R, Kaumaya PTP, Jones C, Williams MV. The EBV-Encoded dUTPase Activates NF-kB through the TLR2 and MyD88-Dependent Signaling Pathway. J Immunol 2009, 182: 851-859 http://www.jimmunol.org/cgi/content/abstract/182/2/851

Kaler P, Godasi BN, Augenlicht L, Klampfer L. The NF-kB/AKT-dependent Induction of Wnt Signaling in Colon Cancer Cells by Macrophages and IL-1B Cancer Microenvironment (2009) 2:69–80 http://www.springerlink.com/content/a746754h10845125/

Kaler P, Augenlicht L, Klampfer L. Macrophage-derived IL-1beta stimulates Wnt signaling and growth of colon cancer cells: a crosstalk interrupted by vitamin D3.Oncogene 2009, 28:3892-38902 http://www.nature.com/onc/journal/v28/n44/abs/onc2009247a.html

Kaler P, Galea V, Augenlicht L, Klampfer L. Tumor Associated Macrophages Protect Colon Cancer Cells from TRAIL-Induced Apoptosis through IL-1beta-Dependent Stabilization of Snail in Tumor Cells.  PLoS One. 2010 5:e11700.  http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.00117...

FACS Analysis

11c-14 FACS plot

FACS analysis of a typical sample stained with antibodies to CD11c vs CD14.  Samples were gated on live cells.

Cytokine Production
  IL1beta IL6 IL10 IL12 IL13 CCL2 CCL3 TNFa
  pg/ml pg/ml pg/ml pg/ml pg/ml pg/ml pg/ml pg/ml
control 1.0 1.2 ND 1.0 24.1 380.0 110.5 32.2
1 ug/mL LPS 6.2 1,900.0 24.0 11.3 118.3 4,260.0 72,900.0 5,380.0
10 ug/mL LPS 11.1 6,300.0 72.0 11.2 112.4 10,365.0 201,200.0 10,685.0
Monocytes were thawed and cultured in 24 well plates at 1 million cells per well Culture medium was collected after 48 hours Cytokines were quantitated by Pierce Biotechnology using their Searchlight Proteome Array™


Phagocytosis

Monocytes were incubated with Alexa Fluor® 595 conjugated zymosan for one hour, fixed and counterstained with Alexa Fluor® 488 conjugated phalloidin. See the phagocytosis protocol here for details of the method.

(Alexa Fluor is a registered trademark of Molecular Probes, Inc.)

Chemotaxis

Monocytes were thawed and placed into Transwells to measure migration in response to formyl-methionine-leucine-phenylalanine. Click here to see the protocol.