Monocytes - Negative Selection

Our Monocytes are isolated by a negative selection process that prevents the activation of cells. Monocytes from Astarte Biologics function as expected:

  • phagocytize yeast
  • produce cytokines
  • migrate in response to chemotactic agents

Under the right culture conditions, monocytes can differentiate into dendritic cells, Langerhans cells or macrophages.


 

Catalog Number Product Name Size Price In Stock
1008-2645JY14 Monocytes - Negatively Selected 5-10 M cells/vial $330.00 6
1009-2646JY14 Monocytes - Negatively Selected 10-20 M cells/vial $550.00 19
1008-2650AU14 Monocytes - Negatively Selected 5-10 M cells/vial $330.00 4
1008-2655AU14 Monocytes - Negatively Selected 5-10 M cells/vial $330.00 4

 

Publications

Publications using Monocytes from Astarte Biologics:

Wang R, Borazjani A, Matthews AT, et al. Identification of palmitoyl protein thioesterase 1 in human THP-1 monocytes/macrophages and characterization of unique biochemical activities for this enzyme. Biochemistry 2013 abstract

RA Barve, MD Zack, D Weiss, et al. Transcriptional profiling and pathway analysis of CSF-1 and IL-34 effects on human monocyte differentiation.  Cytokine, 2013 abstract

Kaler P, Augenlicht L, Klampfer L. Activating mutations in ß-catenin in colon cancer cells alter their interaction with macrophages; the role of snail PLOS ONE 2012. http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0045462

Cai S, Batra S, Wakamatsu N, et al. NLRC4 Inflammasome-mediated production of IL-1ß modulates mucosal immunity in the lung against gram-negative bacterial infection. J Immunol 2012. http://www.jimmunol.org/content/early/2012/04/30/jimmunol.1200195.abstract

AV Finn, M Nakano, R Polavarapu, et al. Hemoglobin Directs Macrophage Differentiation and Prevents Foam Cell Formation in Human Atherosclerotic Plaques J Am Coll Cardiol published online 7 December 2011.  http://content.onlinejacc.org/cgi/content/abstract/j.jacc.2011.10.852v1

K Subbaramaiah, LR Howe, P Bhardwaj, et al. Obesity is associated with inflammation and elevated aromatase expression in the mouse mammary gland Cancer Prev Res March 2011 4; 329

Xu M, et al. Hepcidin induces HIV-1 transcription inhibited by ferroportin Retrovirology 2010,7:104    full text at Wiley

Ariza M, Glaser R, Kaumaya PTP, Jones C, Williams MV. The EBV-Encoded dUTPase Activates NF-kB through the TLR2 and MyD88-Dependent Signaling Pathway. J Immunol 2009, 182: 851-859 http://www.jimmunol.org/cgi/content/abstract/182/2/851

Kaler P, Godasi BN, Augenlicht L, Klampfer L. The NF-kB/AKT-dependent Induction of Wnt Signaling in Colon Cancer Cells by Macrophages and IL-1B Cancer Microenvironment (2009) 2:69–80 http://www.springerlink.com/content/a746754h10845125/

Kaler P, Augenlicht L, Klampfer L. Macrophage-derived IL-1beta stimulates Wnt signaling and growth of colon cancer cells: a crosstalk interrupted by vitamin D3.Oncogene 2009, 28:3892-38902 http://www.nature.com/onc/journal/v28/n44/abs/onc2009247a.html

Kaler P, Galea V, Augenlicht L, Klampfer L. Tumor Associated Macrophages Protect Colon Cancer Cells from TRAIL-Induced Apoptosis through IL-1beta-Dependent Stabilization of Snail in Tumor Cells.  PLoS One. 2010 5:e11700.  http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.00117...

FACS Analysis

11c-14 FACS plot

FACS analysis of a typical sample stained with antibodies to CD11c vs CD14.  Samples were gated on live cells.

Cytokine Production
  IL1beta IL6 IL10 IL12 IL13 CCL2 CCL3 TNFa
  pg/ml pg/ml pg/ml pg/ml pg/ml pg/ml pg/ml pg/ml
control 1.0 1.2 ND 1.0 24.1 380.0 110.5 32.2
1 ug/mL LPS 6.2 1,900.0 24.0 11.3 118.3 4,260.0 72,900.0 5,380.0
10 ug/mL LPS 11.1 6,300.0 72.0 11.2 112.4 10,365.0 201,200.0 10,685.0
Monocytes were thawed and cultured in 24 well plates at 1 million cells per well Culture medium was collected after 48 hours Cytokines were quantitated by Pierce Biotechnology using their Searchlight Proteome Array™


Phagocytosis

Monocytes were incubated with Alexa Fluor® 595 conjugated zymosan for one hour, fixed and counterstained with Alexa Fluor® 488 conjugated phalloidin. See the phagocytosis protocol here for details of the method.

(Alexa Fluor is a registered trademark of Molecular Probes, Inc.)

Chemotaxis

Monocytes were thawed and placed into Transwells to measure migration in response to formyl-methionine-leucine-phenylalanine. Click here to see the protocol.