Peripheral blood mononuclear cells are collected from healthy donors by leukapheresis and further purified on ficoll-sodium metrizoate density gradients. The cells are washed extensively and frozen in a solution of 10% DMSO, 2% human serum albumin in phosphate buffered saline. Donors are tested for the blood borne pathogens HIV-1/ 2 Antibody, Hepatitis B Surface Antigen and Core Antibody, HCV Antibody, HTLV-I/II, syphilis, NAT HIV/HCV, NAT WNV and found to be negative. Cells should still be handled as if potentially infectious following biosafety level 2 procedures.
Our PBMCs are available in 3 sizes:
| Cells/Vial | Price |
| 5-10M | $75.00 |
| 20-49M | $150.00 |
| 50-99M | $200.00 |
* Hover over Donor to view donor details
| Catalog Number | Product Name | Donor | Size | Price | In Stock | ||||||||||||||||||||||||||||||||||||||||||
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| 1000-1705OC12 | PBMC 20M |
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20-49 M cells/vial | $150.00 | 2 |
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| 1001-1704OC12 | PBMC 50M |
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50-99 M cells/vial | $200.00 | 2 |
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| 1000-2097AP13 | PBMC 20M |
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20-49 M cells/vial | $150.00 | 20 |
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| 1001-2096AP13 | PBMC 50M |
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50-99 M cells/vial | $200.00 | 12 |
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| 1001-1820DE12 | PBMC 50M |
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50-99 M cells/vial | $200.00 | 5 |
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| 1007-1918JA13 | PBMC 5M |
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5-10 M cells/vial | $75.00 | 1 |
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| 1001-1911JA13 | PBMC 50M |
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50-99 M cells/vial | $200.00 | 24 |
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| 10050-1327MY12 | Normal Plasma |
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1 mL/vial | $150.00 | 4 |
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| 1007-1779NV12 | PBMC 5M |
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5-10 M cells/vial | $50.00 | 198 |
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| 1007-1869DE12 | PBMC 5M |
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5-10 M cells/vial | $75.00 | 1 |
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| 1001-1870DE12 | PBMC 50M |
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50-99 M cells/vial | $200.00 | 1 |
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| 1001-1961FE13 | PBMC 50M |
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50-99 M cells/vial | $200.00 | 17 |
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| 1000-1619AU12 | PBMC 20M |
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20-49 M cells/vial | $150.00 | 2 |
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| 1007-1901JA13 | PBMC 5M |
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5-10 M cells/vial | $75.00 | 4 |
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| 1007-1854DE12 | PBMC 5M |
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5-10 M cells/vial | $75.00 | 9 |
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| 1007-1767NV12 | PBMC 5M |
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5-10 M cells/vial | $75.00 | 20 |
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| 1000-1766NV12 | PBMC 20M |
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20-49 M cells/vial | $150.00 | 13 |
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| 1001-1765NV12 | PBMC 50M |
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50-99 M cells/vial | $200.00 | 26 |
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| 1001-1866DE12 | PBMC 50M |
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50-99 M cells/vial | $200.00 | 11 |
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Storage
Store in the vapor phase of liquid nitrogen (< minus 120°C). Product may be stored at < minus 75°C for several days but cell viability will decrease with extended storage.
Testing
Cell count, cell viability and antigen responses are reported on each lot.
FACS
Publications
Publications using PBMC from Astarte Biologics
White RA, et al. Digital PCR provides absolute quantitation of viral load for an occult RNA virus. J Virol Methods, 2011 doi:10.1016/j.jviromet.2011.09.017
El-Hage N, et al. HIV-1 co-infection and Morphine Co-Exposure Severely Dysregulate HCV-induced hepatic pro-inflamatory cytokine release and free radical production: increased pathogenesis coincides with uncoordinated host-defenses. J Virology 2011. published online ahead of print, Sept 7, 2011 abstract
Vuletic S, et al. PLTP Regulates STAT3 and NF [kappa] B in Differentiated THP-1 cells and human monocyte-derived macrophages. Biochim Biophys Acta 2011, abstract
Debebe Z, et al. Iron Chelators of the DpT and BpT Series Inhibit HIV-1 Transcription: Identification of novel cellular targets - Iron, cdk2 and cdk9. Mol. Pharmacology 2010, full text
Xiong G, et al. Novel cancer vaccine based on genes of Salmonella pathogenicity island 2. Int J Cancer 2010,126:2622-2634. abstract
Song L, et al. Vα24-invariant NKT cells mediate antitumor activity via killing of tumor-associated macrophages. J Clin Invest 2009, 119:1524-1536 www.pubmedcentral.nih.gov/articlerender.fcgi
Klinghoffer RA, Frazier J, Annis J, Berndt JD, Roberts BS, et al. 2009 A Lentivirus-Mediated Genetic Screen Identifies Dihydrofolate Reductase (DHFR) as a Modulator of β-Catenin/GSK3 Signaling. PLoS ONE 4(9): e6892. doi:10.1371/journal.pone.0006892 http://www.plosone.org/article
Song L, et al. Oncogene MYCN regulates localization of NKT cells to the site of disease in neuroblastoma J Clin Invest 2007, 117:2702-2712. full text