Peripheral blood mononuclear cells are collected from healthy donors by leukapheresis and further purified on ficoll-sodium metrizoate density gradients. The cells are washed extensively and frozen in a solution of 10% DMSO, 2% human serum albumin in phosphate buffered saline. Donors are tested for the blood borne pathogens HIV-1/ 2 Antibody, Hepatitis B Surface Antigen and Core Antibody, HCV Antibody, HTLV-I/II, syphilis, NAT HIV/HCV, NAT WNV and found to be negative. Cells should still be handled as if potentially infectious following biosafety level 2 procedures.

Our PBMCs are available in 3 sizes:

Storage

Store in the vapor phase of liquid nitrogen (< minus 120°C). Product may be stored at < minus 75°C for several days but cell viability will decrease with extended storage.

Testing

Cell count, cell viability and antigen responses are reported on each lot.

FACS

Publications

Publications using PBMC from Astarte Biologics

Xiong G, et al. Novel cancer vaccine based on genes of Salmonella pathogenicity island 2 Intl J Cancer 2010, 126:2622-2634 full text at Wiley

L. Song,  et al.  Vα24-invariant NKT cells mediate antitumor activity via killing of tumor-associated macrophages.  J Clin Invest 2009, 119:1524-1536     www.pubmedcentral.nih.gov/articlerender.fcgi 

Klinghoffer RA, Frazier J, Annis J, Berndt JD, Roberts BS, et al. 2009 A Lentivirus-Mediated Genetic Screen Identifies Dihydrofolate Reductase (DHFR) as a Modulator of β-Catenin/GSK3 Signaling. PLoS ONE 4(9): e6892. doi:10.1371/journal.pone.0006892   http://www.plosone.org/article