Peripheral Blood Mononuclear Cells

Peripheral blood mononuclear cells are collected from healthy donors by leukapheresis and further purified on ficoll-sodium metrizoate density gradients. The cells are washed extensively and frozen in a solution of 10% DMSO, 2% human serum albumin in phosphate buffered saline. Donors are tested for the blood borne pathogens HIV-1/ 2 Antibody, Hepatitis B Surface Antigen and Core Antibody, HCV Antibody, HTLV-I/II, syphilis, NAT HIV/HCV, NAT WNV and found to be negative. Cells should still be handled as if potentially infectious following biosafety level 2 procedures.

Our PBMCs are available in 3 sizes:

Cells/Vial Price
5-10M $75.00
20-49M $150.00
50-99M $200.00

Catalog NumberProduct NameDonorBld TypeM1CMVTTLPSPHASizePrice
1000-284NV09PBMC 20M

 

Donor #: 003
Blood Type: A pos
Age: 56
Gender: M
Ethnicity: Caucasian
M1 Peptide:neg
CMV Antigens:pos
Tetanus Toxoid:neg
LPS:N/T
PHA:N/T
HLA-A*0201*0201
HLA-B*2705*14
HLA-DRß1*1501*14

Available Lots

1001-66JY0650-99 M cells/vial$200.00
1000-284NV0920-50 M cells/vial$150.00
A posnegposnegN/TN/T20-50 M cells/vial$150.00
1000-337FE10PBMC 20 M

 

Donor #: 089
Blood Type: B neg
Age: 47
Gender: M
Ethnicity: African American
M1 Peptide:N/T
CMV Antigens:neg
Tetanus Toxoid:neg
LPS:neg
PHA:pos

Genetic typing can be performed for an additional charge.

Available Lots

1000-337FE1020-49 M cells/vial$150.00
B negN/Tnegnegnegpos20-49 M cells/vial$150.00
1000-385AP10PBMC 20M

 

Donor #: 098
Blood Type: O pos
Age: 38
Gender: M
Ethnicity: African American
M1 Peptide:N/T
CMV Antigens:pos
Tetanus Toxoid:pos
LPS:pos
PHA:pos

Genetic typing can be performed for an additional charge.

Available Lots

1007-386AP105-10 M cells/vial$75.00
1000-385AP1020-49 M cells/vial$150.00
1001-383AP1050-100 M cells/vial$200.00
O posN/Tpospospospos20-49 M cells/vial$150.00
1000-410AP10PBMC 20M

 

Donor #: 033
Blood Type: B pos
Age: 30
Gender: M
Ethnicity:
M1 Peptide:N/T
CMV Antigens:neg
Tetanus Toxoid:N/T
LPS:pos
PHA:pos

Genetic typing can be performed for an additional charge.

Available Lots

1001-409AP1050-100 M cells/vial$200.00
1000-410AP1020-49 M cells/vial$150.00
1007-411AP105-10 M cells/vial$75.00
B posN/TnegN/Tpospos20-49 M cells/vial$150.00
1001-139OC08PBMC 50M

 

Donor #: 040
Blood Type: B pos
Age: 38
Gender: M
Ethnicity: African American
M1 Peptide:N/T
CMV Antigens:neg
Tetanus Toxoid:pos
LPS:pos
PHA:pos

Genetic typing can be performed for an additional charge.

Available Lots

1001-139OC0850-99 M cells/vial$200.00
1001-152DE0850-99 M cells/vial$200.00
B posN/Tnegpospospos50-99 M cells/vial$200.00
1001-152DE08PBMC 50M

 

Donor #: 040
Blood Type: B pos
Age: 38
Gender: M
Ethnicity: African American
M1 Peptide:N/T
CMV Antigens:neg
Tetanus Toxoid:pos
LPS:pos
PHA:pos

Genetic typing can be performed for an additional charge.

Available Lots

1001-139OC0850-99 M cells/vial$200.00
1001-152DE0850-99 M cells/vial$200.00
B posN/Tnegpospospos50-99 M cells/vial$200.00
1001-362MA10PBMC 50 M

 

Donor #: 097
Blood Type: A neg
Age: 41
Gender: M
Ethnicity: African American
M1 Peptide:N/T
CMV Antigens:N/T
Tetanus Toxoid:N/T
LPS:N/T
PHA:N/T

Genetic typing can be performed for an additional charge.

Available Lots

1001-362MA1050-100 M cells/vial$200.00
A negN/TN/TN/TN/TN/T50-100 M cells/vial$200.00
1001-383AP10PBMC 50 M

 

Donor #: 098
Blood Type: O pos
Age: 38
Gender: M
Ethnicity: African American
M1 Peptide:N/T
CMV Antigens:pos
Tetanus Toxoid:pos
LPS:pos
PHA:pos

Genetic typing can be performed for an additional charge.

Available Lots

1007-386AP105-10 M cells/vial$75.00
1000-385AP1020-49 M cells/vial$150.00
1001-383AP1050-100 M cells/vial$200.00
O posN/Tpospospospos50-100 M cells/vial$200.00
1001-409AP10PBMC 50 M

 

Donor #: 033
Blood Type: B pos
Age: 30
Gender: M
Ethnicity:
M1 Peptide:N/T
CMV Antigens:neg
Tetanus Toxoid:N/T
LPS:pos
PHA:pos

Genetic typing can be performed for an additional charge.

Available Lots

1001-409AP1050-100 M cells/vial$200.00
1000-410AP1020-49 M cells/vial$150.00
1007-411AP105-10 M cells/vial$75.00
B posN/TnegN/Tpospos50-100 M cells/vial$200.00
1001-418MY10PBMC 50 M

 

Donor #: 091
Blood Type: AB pos
Age: 40
Gender: M
Ethnicity: African American
M1 Peptide:N/T
CMV Antigens:N/T
Tetanus Toxoid:pos
LPS:pos
PHA:pos

Genetic typing can be performed for an additional charge.

Available Lots

1001-418MY1050-99 M cells/vial$200.00
1001-454JN1050-100 M cells/vial$200.00
1001-486JY1050-100 M/vial$200.00
AB posN/TN/Tpospospos50-99 M cells/vial$200.00
1001-434MY10PBMC 50M

 

Donor #: 105
Blood Type: AB pos
Age: 54
Gender: M
Ethnicity: Caucasian
M1 Peptide:N/T
CMV Antigens:N/T
Tetanus Toxoid:N/T
LPS:N/T
PHA:N/T

Genetic typing can be performed for an additional charge.

Available Lots

1001-434MY1050-100 M cells/vial$200.00
AB posN/TN/TN/TN/TN/T50-100 M cells/vial$200.00
1001-441MY10PBMC 50 M

 

Donor #: 096
Blood Type: A neg
Age: 35
Gender: F
Ethnicity: Caucasian
M1 Peptide:N/T
CMV Antigens:neg
Tetanus Toxoid:pos
LPS:pos
PHA:pos
HLA-A*0201*23
HLA-B*45*07
HLA-C*07*06
HLA-DRB1*07*15

Available Lots

1001-441MY1050-99 M cells/vial$200.00
A negN/Tnegpospospos50-99 M cells/vial$200.00
1001-454JN10PBMC 50 M

 

Donor #: 091
Blood Type: AB pos
Age: 40
Gender: M
Ethnicity: African American
M1 Peptide:N/T
CMV Antigens:N/T
Tetanus Toxoid:pos
LPS:pos
PHA:pos

Genetic typing can be performed for an additional charge.

Available Lots

1001-418MY1050-99 M cells/vial$200.00
1001-454JN1050-100 M cells/vial$200.00
1001-486JY1050-100 M/vial$200.00
AB posN/TN/Tpospospos50-100 M cells/vial$200.00
1001-486JY10PBMC 50M

 

Donor #: 091
Blood Type: AB pos
Age: 40
Gender: M
Ethnicity: African American
M1 Peptide:N/T
CMV Antigens:N/T
Tetanus Toxoid:pos
LPS:pos
PHA:pos

Genetic typing can be performed for an additional charge.

Available Lots

1001-418MY1050-99 M cells/vial$200.00
1001-454JN1050-100 M cells/vial$200.00
1001-486JY1050-100 M/vial$200.00
AB posN/TN/Tpospospos50-100 M/vial$200.00
1001-66JY06PBMC 50M

 

Donor #: 003
Blood Type: A pos
Age: 56
Gender: M
Ethnicity: Caucasian
M1 Peptide:neg
CMV Antigens:pos
Tetanus Toxoid:neg
LPS:N/T
PHA:N/T
HLA-A*0201*0201
HLA-B*2705*14
HLA-DRß1*1501*14

Available Lots

1001-66JY0650-99 M cells/vial$200.00
1000-284NV0920-50 M cells/vial$150.00
A posnegposnegN/TN/T50-99 M cells/vial$200.00
1007-382AP10PBMC 5-10M

 

Donor #: 038
Blood Type: B pos
Age: 31
Gender: M
Ethnicity: African American
M1 Peptide:N/T
CMV Antigens:pos
Tetanus Toxoid:pos
LPS:pos
PHA:pos
HLA-A*01*03
HLA-B*44*57
HLA-Cw*06w*04

Available Lots

1007-382AP105-10 M cells/vial$75.00
B posN/Tpospospospos5-10 M cells/vial$75.00
1007-386AP10PBMC 5M

 

Donor #: 098
Blood Type: O pos
Age: 38
Gender: M
Ethnicity: African American
M1 Peptide:N/T
CMV Antigens:pos
Tetanus Toxoid:pos
LPS:pos
PHA:pos

Genetic typing can be performed for an additional charge.

Available Lots

1007-386AP105-10 M cells/vial$75.00
1000-385AP1020-49 M cells/vial$150.00
1001-383AP1050-100 M cells/vial$200.00
O posN/Tpospospospos5-10 M cells/vial$75.00
1007-411AP10PBMC 5M

 

Donor #: 033
Blood Type: B pos
Age: 30
Gender: M
Ethnicity:
M1 Peptide:N/T
CMV Antigens:neg
Tetanus Toxoid:N/T
LPS:pos
PHA:pos

Genetic typing can be performed for an additional charge.

Available Lots

1001-409AP1050-100 M cells/vial$200.00
1000-410AP1020-49 M cells/vial$150.00
1007-411AP105-10 M cells/vial$75.00
B posN/TnegN/Tpospos5-10 M cells/vial$75.00
1007-485JY10PBMC 5M

 

Donor #: 112
Blood Type: O pos
Age: 30
Gender: F
Ethnicity: Caucasian
M1 Peptide:N/T
CMV Antigens:pos
Tetanus Toxoid:pos
LPS:pos
PHA:pos
HLA-A*24*02
HLA-B*44*18
HLA-C*12*05
HLA-DRB1*1501*0101
HLA-DQB1*06*05

Available Lots

1007-485JY105-10 M cells/vial$75.00
O posN/Tpospospospos5-10 M cells/vial$75.00
1007-499AU10PBMC 5 M

 

Donor #: 142
Blood Type: A pos
Age: 47
Gender: M
Ethnicity: Caucasian
M1 Peptide:N/T
CMV Antigens:neg
Tetanus Toxoid:pos
LPS:pos
PHA:pos
HLA-A*68*31
HLA-B*51*35
HLA-C*16*04
HLA-DRb1*15*01

Available Lots

1007-499AU105-10 M cells/vial$75.00
A posN/Tnegpospospos5-10 M cells/vial$75.00

PBMC Advanced Search

Storage

Store in the vapor phase of liquid nitrogen (< minus 120°C). Product may be stored at < minus 75°C for several days but cell viability will decrease with extended storage.

Testing

Cell count, cell viability and antigen responses are reported on each lot.

FACS

 

Publications

Publications using PBMC from Astarte Biologics

Xiong G, et al. Novel cancer vaccine based on genes of Salmonella pathogenicity island 2 Intl J Cancer 2010, 126:2622-2634 full text at Wiley

L. Song,  et al.  Vα24-invariant NKT cells mediate antitumor activity via killing of tumor-associated macrophages.  J Clin Invest 2009, 119:1524-1536     www.pubmedcentral.nih.gov/articlerender.fcgi 

Klinghoffer RA, Frazier J, Annis J, Berndt JD, Roberts BS, et al. 2009 A Lentivirus-Mediated Genetic Screen Identifies Dihydrofolate Reductase (DHFR) as a Modulator of β-Catenin/GSK3 Signaling. PLoS ONE 4(9): e6892. doi:10.1371/journal.pone.0006892   http://www.plosone.org/article

 FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE.