Peripheral blood mononuclear cells are collected from healthy donors by leukapheresis and further purified on ficoll-sodium metrizoate density gradients. The cells are washed extensively and frozen in a solution of 10% DMSO, 2% human serum albumin in phosphate buffered saline. Donors are tested for the blood borne pathogens HIV-1/ 2 Antibody, Hepatitis B Surface Antigen and Core Antibody, HCV Antibody, HTLV-I/II, syphilis, NAT HIV/HCV, NAT WNV and found to be negative. Cells should still be handled as if potentially infectious following biosafety level 2 procedures.

Storage

Store in the vapor phase of liquid nitrogen (< minus 120°C). Product may be stored at < minus 75°C for several days but cell viability will decrease with extended storage.

Testing

Cell count, cell viability, HLA type and antigen responses are reported on each lot.

FACS