Peripheral blood mononuclear cells are collected from healthy donors by leukapheresis and further purified on ficoll-sodium metrizoate density gradients. The cells are washed extensively and frozen in a solution of 10% DMSO, 2% human serum albumin in phosphate buffered saline. Donors are tested for the blood borne pathogens HIV-1/ 2 Antibody, Hepatitis B Surface Antigen and Core Antibody, HCV Antibody, HTLV-I/II, syphilis, NAT HIV/HCV, NAT WNV and found to be negative. Cells should still be handled as if potentially infectious following biosafety level 2 procedures.
Storage
Store in the vapor phase of liquid nitrogen (< minus 120°C). Product may be stored at < minus 75°C for several days but cell viability will decrease with extended storage.
Testing
Cell count, cell viability, HLA type and antigen responses are reported on each lot.
| Catalog Number | Cells/Vial | Price |
| 1007 | 5-10M | $75.00 |
| 1000 | 20-49M | $150.00 |
| 1001 | 50-99M | $200.00 |
FACS